Tasa de fertilización, desarrollo y calidad de embriones bovinos Holstein producidos in vitro con semen sexado y adición de IGF-I
Fertilization rate, development and quality of Holstein bovine embryos produced in vitro with sexed semen and addition of IGF-I
DOI:
https://doi.org/10.54167/tch.v9i3.586Palabras clave:
IGF-I, embriones, fertilización in vitro, semen sexadoResumen
La adición de IGF-I a los medios de fertilización in vitro y cultivo de embriones ha sido propuesta como una forma de imitar las señales maternas de la gestación temprana en el útero. El objetivo del presente estudio fue evaluar la adición del factor de crecimiento similar a la insulina tipo-I (IGF-I) sobre la tasa de fertilización con semen sexado (SS) y de blastocistos bovinos producidos in vitro, así como la calidad embrionaria. Se utilizó SS de tres toros Holstein para la fertilización in vitro (FIV) de ovocitos obtenidos de ovarios recolectados en rastro, para lo cual se agregó IGF-I (100 ng/ml) en los medios de fertilización y desarrollo embrionario a diferentes tiempos: T1 (IGF-I, d 0-7; n= 393); T2 (IGF-I, d 0-3; n= 394); T3 (IGF-I, d 3-7; n= 394); y T4 (sin IGF-I o grupo control; n= 394). Se evaluó el porcentaje de fertilización al día tres de incubación y el día siete se evaluó la calidad y estadio de los blastocistos. Los datos se analizaron con el procedimiento CATMOD de SAS (SAS Inst. Inc., Cary, NC), ajustando un modelo que incluyó el efecto de tratamiento (con y sin IGF-I, para tasa de fertilización; y T1, T2, T3 y T4 para otras variables). La adición de IGF-I al medio afectó la tasa de fertilización (34 vs 42 %; P < 0.05) y no hubo efecto (P > 0.05) de tratamiento para la tasa de blastocistos/ovocitos fertilizados, ni para calidad de los blastocistos. Se concluye que bajo las condiciones del presente estudio, la adición de IGF-I a los medios de fertilización y cultivo in vitro no tiene un efecto benéfico para la producción de embriones, pero sí afecta la fertilidad del semen sexado.
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