Comparación de la eficiencia de transformación entre diferentes cepas de E. coli
Comparison of transformation efficiency in different E. coli strains
DOI:
https://doi.org/10.54167/tch.v13i2.434Palabras clave:
E. coli, transformación, thermal shock, MgCl2/ CaCl2 methodResumen
La transformación es la introducción y expresión de ADN exógeno por células bacterianas. La eficiencia de la transformación puede medirse en unidades formadoras de colonia/ml (UFC/ml) y es susceptible al método utilizado, a la cepa bacteriana utilizada para la expresión y al propio vector. En este trabajo se busca evaluar las diferencias en la eficiencia de transformación de dos plasmidos de expresión (pExp-Lib y pSF-CMV- Ub-puro-SV40 Ori Sbfl), en cuatro cepas diferentes de E. coli (DH5a, BL21, XL1-Blue y TG1) utilizando un método de preparación de células competentes basado en el uso de MgCl2/CaCl2. En todas las cepas utilizadas, el crecimiento bacteriano y la eficiencia de transformación fueron mayores para las cepas con el vector pExp, a excepción de BL21, donde la eficiencia fue más elevada para el vector pSF.
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