Use of Supercritical fluid extraction (SFE) as a Sustainable Strategy for Polyphenol Recovery from Salvilla (Buddleja scordioides Kunth)
DOI:
https://doi.org/10.54167/tch.v20i1.2175Keywords:
salvilla, extraction, supercritical fluids, phenolic acids, flavonoidsAbstract
Emerging extraction technologies gained position due to their advantages in obtaining high-quality metabolites. Supercritical fluid extraction (SFE) recovers bioactive molecules while preserving their integrity. In this study, SFE was applied to Buddleja scordioides Kunth (salvilla) bush leaves, a species rich in phenolic compounds with medicinal properties. A central composite design of three factors was used: co‑solvent percentage (ethanol), pressure, and extraction time. Extracts were evaluated for yield, total flavonoid content, antioxidant activity (ORAC and ABTS) and quantification of phenolic compounds by UPLC-PDA-ESI-MS/MS. Extraction yields ranged from 3.63-6.27 %, total phenolic content varied from 219.17-314.72 μg CE/mg extract. Antioxidant activity was ranged from 53.54–81.88 mmol TE/mg of salvilla leaves extract (SE) for ABTS and 78.69-185.40 mmol TE/mg SE for ORAC. The total phenolic content determined by UPLC-PDA-ESI-MS/MS was ranged from 4188.1-13008.2 ng/mg SE with the co‑solvent as the most influencer factor. According to the general linear model (GLM), the central points (120 min, 150 bar, 20 % co‑solvent) yielded the highest results. This work is original in its application of SFE, to improve the extraction of bioactive molecules. This work provides novelty by applying SFE, to enhance the extraction and quality of bioactive compounds. These results enhance the SFE as a sustainable strategy for the valorization of medicinal plants.
DOI: https://doi.org/10.54167/tch.v20i1.2175
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