Comparison between different pretreatment methods to obtain fermentable sugars from amaranth stubble
DOI:
https://doi.org/10.54167/tch.v19i4.1765Keywords:
amaranth, stubble, pretreatment, reducing sugars, delignificationAbstract
Agro-industrial residues are gaining increasing importance for their use in various fermentation processes, including the production of substances with the biological activity of industrial value. Fermentable sugars (FS) are a key group of compounds for these processes. However, a series of steps must be carried out to facilitate their release. Pretreatment is a crucial step in obtaining these compounds. Therefore, the objective of this research work was to find a suitable method for delignification of amaranth stubble and subsequent production of fermentable sugars. For this purpose, five pretreatment methods were tested, which were classified as follows: physical (I. steam and II. ultrasound), chemical (III. 3.5N NaOH), and combined (IV. 3.5N NaOH + steam and V. NaOH (2, 2.5, 3, 3.5 N) + ultrasound). The Miller method (DNS) for reducing sugars was used to determine the concentration of FS, which would indicate the degradation of lignocellulose. This was corroborated by FTIR analysis. The methods that obtained the highest concentration of FS were the physical pretreatment (II. ultrasound) and the combined pretreatment (V. 3.5 N + ultrasound). Once the pretreatment method was defined, the saccharification process was carried out through conventional enzymatic hydrolysis and ultrasound-assisted hydrolysis, with the latter showing greater efficiency.
DOI: https://doi.org/10.54167/tch.v19i4.1765Downloads
References
Araújo, D., Vilarinho, M., & Machado, A. (2019). Effect of combined dilute-alkaline and green pretreatments on corncob fractionation: Pretreated biomass characterization and regenerated cellulose film production. Industrial Crops and Products, 141: 111785. https://doi.org/10.1016/j.indcrop.2019.111785
Arce, M. B. (2011). Obtención de compuestos biofuncionales a partir del material lignocelulósico de Amaranthus hypochondriacus L. (Tesis de Maestría, Universidad de Autónoma Metropolitana). https://doi.org/10.24275/uami.2b88qc43c
Arias-Giraldo, S., & López-Velasco, D. M. (2019). Reacciones químicas de los azúcares simples empleados en la industria alimentaria. Lámpsakos, 22: 123–136. https://doi.org/10.21501/21454086.3252
Bressani, R. (1989). The proteins of grain amaranth. Food Reviews International, 5(1), 13–38. https://doi.org/10.1080/87559128909540843
Cardona, E. M., Rios, J. A., Peña, J. D., & Rios, L. A. (2013). Pretratamiento Alcalino de Pasto Elefante (Pennisetum sp) y King Grass (Pennisetum hybridum) Cultivados en Colombia para la Producción de Bioetanol. Información Tecnológica, 24(5): 69–80. https://doi.org/10.4067/S0718-07642013000500009
Chen, Y., Jiang, S., Yuan, H., Zhou, Q., & Gu, G. (2007). Hydrolysis and acidification of waste activated sludge at different pHs. Water Research, 41(3): 683–689. https://doi.org/10.1016/j.watres.2006.07.030
Chu, Q., Wang, R., Tong, W., Jin, Y., Hu, J., & Song, K. (2020). Improving Enzymatic Saccharification and Ethanol Production from Hardwood by Deacetylation and Steam Pretreatment: Insight into Mitigating Lignin Inhibition. ACS Sustainable Chemistry & Engineering, 8(49): 17967–17978. https://doi.org/10.1021/acssuschemeng.0c05583
Cruz-Cardona, Y., Cadena-Chamorro, E., & Arango-Tobón, J. C. (2019). Procesamiento de la Cascarilla de Cebada Cervecera por Vía Enzimática para la Obtención de AF. Información Tecnológica, 30(4): 41–50. https://doi.org/10.4067/S0718-07642019000400041
Dewi, R.-K., Hudha, M. I., Rauda, A., & Taulusia, S. (2019). Chemical and Physical Process Combinations: Microwave in Lignin Degradation of Pecan Shells as Alternative Fuel Raw Materials. Atlantis Highlights in Chemistry and Pharmaceutical Sciences, 1: 63–67. https://www.atlantis-press.com/article/125929209
García-Pereyra, J., Valdés-Lozano, C., Olivares-Saenz, E., Alvarado-Gómez, O., Alejandre-Iturbide, G., Salazar-Sosa, E., & Medrano-Roldán, H. (2009). Rendimiento de grano y calidad del forraje de amaranto (Amaranthus spp.) cultivado a diferentes densidades en el noreste de México. Phyton (B. Aires), 78(1): 53–60. https://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000100010
García-Pérez, J. V. (2007). Contribución al estudio de la aplicación de ultrasonidos de potencia en el secado convectivo de alimentos. (Tesis doctoral) Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/1890
Gasparotto, J. M., Werle, L. B., Mainardi, M. A., Foletto, E. L., Kuhn, R. C., Jahn, S. L., & Mazutti, M. A. (2015). Ultrasound-assisted hydrolysis of sugarcane bagasse using cellulolytic enzymes by direct and indirect sonication. Biocatalysis and Agricultural Biotechnology, 4(4): 480–485. https://doi.org/10.1016/j.bcab.2015.07.006
Ghaffar, S. H., & Fan, M. (2013). Structural analysis for lignin characteristics in biomass straw. Biomass and Bioenergy, 5: 264–279. https://doi.org/10.1016/j.biombioe.2013.07.015
Gómez, E. A., Ríos, L. A. & Peña, J. D. (2013). Effect of Pretreatment of Woody Biomass on Ethanol Yield. Información tecnológica, 24(5): 113-122. http://dx.doi.org/10.4067/S0718-07642013000500013
Gong, Z., Wang, X., Yuan, W., Wang, Y., Zhou, W., Wang, G., & Liu, Y. (2020). Fed-batch enzymatic hydrolysis of alkaline organosolv-pretreated corn stover facilitating high concentrations and yields of fermentable sugars for microbial lipid production. Biotechnology for Biofuels, 13: 13. https://doi.org/10.1186/s13068-019-1639-9
Haldar, D., & Purkait, M. K. (2021). A review on the environment-friendly emerging techniques for pretreatment of lignocellulosic biomass: Mechanistic insight and advancements. Chemosphere, 264(Part 2): 128523. https://doi.org/10.1016/j.chemosphere.2020.128523
Harmsen, PFH, Huijgen, W, Bermudez, L & Bakker, R. (2010). Literature review of physical and chemical pretreatment processes for lignocellulosic biomass. Report / Wageningen UR, Food & Biobased Research, no. 1184, Wageningen UR Food & Biobased Research, Wageningen. https://edepot.wur.nl/150289
Hernández-Beltrán, J. U., Hernández-De Lira, I. O., Cruz-Santos, M. M., Saucedo-Luevanos, A., Hernández-Terán, F., & Balagurusamy, N. (2019). Insight into Pretreatment Methods of Lignocellulosic Biomass to Increase Biogas Yield: Current State, Challenges, and Opportunities. Applied Sciences, 9(18): 3721. https://doi.org/10.3390/app9183721
Huang, J., Khan, M. T., Perecin, D., Coelho, S. T., & Zhang, M. (2020). Sugarcane for bioethanol production: Potential of bagasse in Chinese perspective. Renewable and Sustainable Energy Reviews, 133: 110296. https://doi.org/10.1016/j.rser.2020.110296
Jiang, L., Wu, Y., Wu, N., Zhong, H., Zhang, Y., Zhao, Z., Li, H., & Zhang, F. (2020). Selective saccharification of microwave-assisted glycerol pretreated corncobs via fast pyrolysis and enzymatic hydrolysis. Fuel, 265: 116965. https://doi.org/10.1016/j.fuel.2019.116965
Lunelli, F. C., Sfalcin, P., Souza, M., Zimmermann, E., Dal Prá, V., Foletto, E. L., Jahn, S. L., Kuhn, R. C., & Mazutti, M. A. (2014). Ultrasound-assisted enzymatic hydrolysis of sugarcane bagasse for the production of fermentable sugars. Biosystems Engineering, 124: 24–28. https://doi.org/10.1016/j.biosystemseng.2014.06.004
Luo, X., Wang, Q., Fang, D., Zhuang, W., Chen, C., Jiang, W., & Zheng, Y. (2018). Modification of insoluble dietary fibers from bamboo shoot shell: Structural characterization and functional properties. International Journal of Biological Macromolecules, 120(Part B): 1461–1467. https://doi.org/10.1016/j.ijbiomac.2018.09.149
Madadi, M., Wang, Y., Zhang, R., Hu, Z., Gao, H., Dan Zhan, Yu, H., Yang, Q., Wang, Y., Tu, Y., Xia, T., & Peng, L. (2022). Integrating mild chemical pretreatments with endogenous protein supplement for complete biomass saccharification to maximize bioethanol production by enhancing cellulases adsorption in novel bioenergy Amaranthus. Industrial Crops and Products, 177: 114471. https://doi.org/10.1016/j.indcrop.2021.114471
Marx, S., Xaba, N., & Chiyanzu, I. (2014). Amaranth lignocellulose as feedstock for bioethanol production: effect of microwave assisted alkaline pretreatment on reducing sugar yield. 22nd European Biomass Conference and Exhibition, 896–900. https://repository.nwu.ac.za/bitstream/handle/10394/16671/Amaranth_lignocellulose_as_feedstock.pdf?sequence=2
Meléndez-Hernández, P. A., Hernández-Beltrán, J. U., Hernández-Escoto, H., & Morales-Rodriguez, R. (2016). Análisis del Pretratamiento de Residuos Lignocelulósicos para La Producción de Biocombustibles y Bioproductos de Alto Valor Agregado. Jóvenes En La Ciencia, 2(1): 508–512. https://www.jovenesenlaciencia.ugto.mx/index.php/jovenesenlaciencia/article/view/1094/727
Méndez-Arango, M. F., López-Sosa, D., Núñez-Gaona, O., & García-Gómez, M. de J. (2019). Evaluación del crecimiento radial sobre forraje de amaranto de hongos filamentosos aislados de hojas de café. Mexican Journal of Biotechnology, 4(2): 47–59. https://doi.org/10.29267/mxjb.2019.4.2.47
Miller, G. L. (1959). Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry, 31(3): 426-428. https://doi.org/10.1021/ac60147a030
Modenbach, A. A., & Nokes, S. E. (2014). Effects of Sodium Hydroxide Pretreatment on Structural Components of Biomass. Transactions of the ASABE, 57(4): 1187–1198. https://doi.org/10.13031/trans.57.10046
Mohapatra, S., Dandapat, S. J., & Thatoi, H. (2017). Physicochemical characterization, modelling and optimization of ultrasono-assisted acid pretreatment of two Pennisetum sp. using Taguchi and artificial neural networking for enhanced delignification. Journal of Environmental Management, 187: 537–549. https://doi.org/10.1016/j.jenvman.2016.09.060
Nagula, K. N. & Pandit, A. B. (2016). Process intensification of delignification and enzymatic hydrolysis of delignified cellulosic biomass using various process intensification techniques including cavitation. Bioresource Technology, 213: 162-168. https://doi.org/10.1016/j.biortech.2016.03.152
Nolasco-Hipólito, C., Ramírez-Isidro, O., Núñez-Gaona, O., Ávila-Alejandre, A. X., Hernández-López, A., & García-Gómez, M. de J. (2022). Antioxidant activity of peptides obtained by enzymatic hydrolysis from proteins of amaranth (Amaranthus hypochondriacus L.) stubble. Agrociencia 56(3): 434-450. https://doi.org/10.47163/agrociencia.v56i3.2800
Patil, R. S., Joshi, S. M., & Gogate, P. R. (2019). Intensification of delignification of sawdust and subsequent enzymatic hydrolysis using ultrasound. Ultrasonics Sonochemistry, 58: 104656. https://doi.org/10.1016/j.ultsonch.2019.104656
Písaříková, B., Peterka, J., Trčková, M., Moudrý, J., Zralý, Z., & Herzig, I. (2006). Chemical Composition of the Above-ground Biomass of Amaranthus cruentus and A. hypochondriacus. Acta Veterinaria Brno, 75(1): 133–138. https://doi.org/10.2754/avb200675010133
Qing, Q., Guo, Q., Zhou, L., Gao, X., Lu, X., & Zhang, Y. (2017). Comparison of alkaline and acid pretreatments for enzymatic hydrolysis of soybean hull and soybean straw to produce fermentable sugars. Industrial Crops and Products, 109: 391–397. https://doi.org/10.1016/j.indcrop.2017.08.051
Reales, J. G., Castaño, H. I., Zapata, J. E. (2016). Evaluación de Tres Métodos de Pretratamiento Químico sobre la Deslignificación de Tallos de Yuca. Información Tecnológica, 27 (3): 11-22. http://dx.doi.org/10.4067/S0718-07642016000300003
Secretaría de Economía [SE]. (2013). NMX-F-607-NORMEX-2013. Alimentos-determinación de cenizas en alimentos-método de prueba. Ciudad de México: SE. https://www.dof.gob.mx/nota_detalle.php?codigo=5311757&fecha=27/08/2013#gsc.tab=0
Secretaría de Economía [SE]. (2012). NOM-155-SCFI-2012. Leche-Denominaciones, especificaciones fisicoquímicas, información comercial y métodos de prueba. Ciudad de México: SE. https://www.dof.gob.mx/normasOficiales/4692/seeco/seeco.htm
Secretaría de Salud [SSA]. (1994). NOM-116-SSA1-1994. Determinación de humedad en alimentos por tratamiento térmico. Ciudad de México: SSA. https://dof.gob.mx/nota_detalle.php?codigo=4728942&fecha=15/08/1994#gsc.tab=0
Secretaría de Salud [SSA]. (1994). NOM-086-SSA1-1994. Alimentos y bebidas no alcohólicas con modificaciones en su composición. Especificaciones nutrimentales. Ciudad de México: SSA. https://dof.gob.mx/nota_detalle.php?codigo=4890075&fecha=26/06/1996#gsc.tab=0
Shah, J. J., & Mohanraj, K. (2014). Comparison of Conventional and Microwave-assisted Synthesis of Benzotriazole Derivatives. Indian Journal of Pharmaceutical Sciences, 76(1): 46–53. https://pmc.ncbi.nlm.nih.gov/articles/PMC4007255/
Subhedar, P. B., & Gogate, P. R. (2016). Use of Ultrasound for Pretreatment of Biomass and Subsequent Hydrolysis and Fermentation. In Solange I. Mussatto (Eds) Biomass Fractionation Technologies for a Lignocellulosic Feedstock Based Biorefinery (pp. 127–149). Elsevier. https://doi.org/10.1016/B978-0-12-802323-5.00006-2
Subhedar, P. B., Ray, P., & Gogate, P. R. (2018). Intensification of delignification and subsequent hydrolysis for the fermentable sugar production from lignocellulosic biomass using ultrasonic irradiation. Ultrasonics Sonochemistry, 40(Part B): 140–150. https://doi.org/10.1016/j.ultsonch.2017.01.030
Torres da Silva, G., Chiarello, L. M., Lima, E. M., Ramos, L. P. (2016). Sono-assisted alkaline pretreatment of sugarcane bagasse for cellulosic ethanol production. Catalysis Today, 269: 21-28. https://doi.org/10.1016/j.cattod.2015.11.023
Vargas-Radillo, J. J., Salazar-Ríos, E., Barrientos-Ramírez, L., Pérez-Centeno, A., Rentería-Urquiza, M., Rodríguez-Rivas, A., Navarro-Arzate, F., & Rutiaga-Quiñones, J. (2016). Pasta blanqueada y AF a partir de médula de bagazo de caña. Madera y Bosques, 21(2): 117-130. https://doi.org/10.21829/myb.2015.212449
Wang, S., Li, F., Zhang, P., Jin, S., Tao, X., Tang, X., Ye, J., Nabi, M. & Wang, H. (2017). Ultrasound assisted alkaline pretreatment to enhance enzymatic saccharification of grass clipping. Energy Conversion and Management. 149: 409–415. https://doi.org/10.1016/j.enconman.2017.07.042
Wu, H., Dai, X., Zhou, S.-L., Gan, Y.-Y., Xiong, Z.-Y., Qin, Y.-H., Ma, J., Yang, L., Wu, Z.-K., Wang, T.-L., Wang, W.-G., & Wang, C.-W. (2017). Ultrasound-assisted alkaline pretreatment for enhancing the enzymatic hydrolysis of rice straw by using the heat energy dissipated from ultrasonication. Bioresource Technology, 241: 70–74. https://doi.org/10.1016/j.biortech.2017.05.090
Xu, J., & Cheng, J. J. (2011). Pretreatment of switchgrass for sugar production with the combination of sodium hydroxide and lime. Bioresource Technology, 102(4): 3861–3868. https://doi.org/10.1016/j.biortech.2010.12.038
Yadav, A., Sharma, V., Tsai, M.-L., Chen, C.-W., Sun, P.-P., Nargotra, P., Wang, J.-X., & Dong, C.-D. (2023). Development of lignocellulosic biorefineries for the sustainable production of biofuels: Towards circular bioeconomy. Bioresource Technology, 381: 129145. https://doi.org/10.1016/j.biortech.2023.129145
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