Técnica de evaluación para damping-off en frijol (Phaseolus vulgaris l.) infectado por Pythium aphanidermatum
Screening technique for damping-off in bean (Phaseolus vulgaris l.) infected by Pythium aphanidermatum
DOI:
https://doi.org/10.54167/tch.v11i2.156Keywords:
seed decay, seedcoat color, mycelium, root rot, damage indexAbstract
A laboratory experiment was conducted with six bean varieties, representing a range in germination capacity and seedcoat colors; the cultivars were sown and infected with a pathogenic oomycete isolate of Pythium aphanidermatum (Edson) Fitzp. The purpose of this study was to develop a seedling screening technique for seed decay and damping-off diseases, which are produced by the pathogenic species Pythium aphanidermatum on cultivated beans (Phaseolus vulgaris L.). A completely randomized design was used, with a factorial arrangement 6 x 2 and three repetitions; Factor A was represented by cultivars: Mantequilla Tropical, Azufrado, Negro Jamapa, Delicias 71, Agramejo and Selección # 4; Factor B consisted of two isolate concentrations, levels 0 and 104 ml-1; experimental unit (EU) was represented by 20 bean seeds, sown on a Petri dish. The findings of this study indicated that the screening technique was successful to cause Pythium diseases and the level of resistance was clearly associated with the cultivar. «Negro Jamapa» (black seedcoat) and «Azufrado» (yellow seedcoat) were the most resistant to the pathogen; on the other hand, «Delicias 71» and «Selección # 4», which have spotty seedcoat, were the most susceptible bean cultivars to Pythium . Low percent germination, high seed decay percentage and root rot were damage and symptoms associated with the susceptible cultivars; moreover, it was also observed the presence of mycellium.
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References
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