Solubilidad de las proteínas del gluten en pan pre-horneado durante el almacenamiento en congelación

Autores/as

DOI:

https://doi.org/10.54167/tch.v19iEspecial.2030

Palabras clave:

red de gluten, tiol libre, desnaturalización de las proteínas, congelación, pan pre-horneado

Resumen

Este estudio investiga cómo la velocidad de congelación y el tiempo de pre-horneado afectan la solubilidad de las proteínas del gluten en el pan, con el objetivo de mejorar la producción de la industria de panificación. Las muestras de pan se pre-hornearon durante 3 y 6 min, se congelaron a velocidad lenta (0,15 °C/min) o rápida (1,45 °C/min) y se almacenaron a -20 °C durante 56 días. Cada 14 días, se analizaron las muestras para medir la solubilidad de las gluteninas de bajo y alto peso molecular (GLU-LMW y GLU-HMW), las gliadinas y el contenido de tioles libres. El análisis estadístico (ANOVA) reveló que el tiempo de pre-horneado, la velocidad de congelación y el tiempo de almacenamiento en congelación influyeron significativamente en el comportamiento de las proteínas (P < 0.01). La solubilidad del GLU-LMW disminuyó 71.2 % con el pre-horneado y el almacenamiento en congelación prolongados, mientras que los niveles de GLU-HMW aumentaron 1.5 % en las mismas condiciones, lo que indica agregación proteica. El contenido de tioles libres disminuyó con un pre-horneado más prolongado 14.5 % (0.35 mol SH/g proteína), pero se conservó mejor con una congelación rápida. En general, la congelación rápida demostró ser más eficaz para preservar las proteínas y la calidad del pan, lo que ofrece información valiosa para optimizar los procesos de producción industrial de pan congelado.

DOI: https://doi.org/10.54167/tch.v19iEspecial.2030

Descargas

Los datos de descarga aún no están disponibles.

Referencias

Armenta-Aispuro, P. G., Rouzaud-Sández, O., López-Franco, Y. L., Lizardi-Mendoza, J., Cárdenas-López, J. L., & Rosell, C. M. (2024). Freezing of baked goods and prepared foods. In S. M. Jafari and H. Rostamabadi (Eds.). Low-Temperature Processing of Food Products. pp. 259-288. Woodhead Publishing, https://doi.org/10.1016/B978-0-12-818733-3.00003-5

American Association of Cereal Chemists. (2000). Approved Methods of the AACC, 10th ed. (Vol. 1 y 2). Minnesota, USA, 2000. https://www.cerealsgrains.org/

Baratto, C. M., Becker, N. B., Gelinski, J. M. L. N., & Silveira, S. M. (2016). Influence of enzymes and ascorbic acid on dough rheology and wheat bread quality. African Journal of Biotechnology, 15(3), 55-61. https://doi.org/10.5897/AJB2015.14931

Bai, N., Guo, X., Xing, J., & Zhu, K. (2022). Effect of freeze-thaw cycles on the physicochemical properties and frying performance of frozen Youtiao dough. Food Chemistry, 386, 132854. https://doi.org/10.1016/j.foodchem.2022.132854

Carboni, A. D., Gómez-Zavaglia, A., Puppo, M.C., & Salinas, M.V. (2022). Effect of freezing wheat dough enriched with calcium salts with/without inulin on bread quality. Foods, 11 (13), 1866. https://doi.org/10.3390/foods11131866

Castillo-Arias, A., Fuenmayor Bobadilla, C. A., & Zuluaga Domínguez, C. M. (2024). Cryoprotectants for Frozen Dough: A Review. Food Biophysics, 19, 18–28. https://doi.org/10.1007/s11483-023-09791-w

Chen, X., Fu, W., Luo, Y., Cui, C., Suppavorasatit, I., & Liang, L. (2021). Protein deamidation to produce processable ingredients and engineered colloids for emerging food applications. Comprehensive Reviews in Food Science and Food Safety, 20(4), 3788–3817. https://doi.org/10.1111/1541-4337.12759

Datamintelligence. (2025). Par-baked bread market size, share análisis, growth trends and forecast report 2025-2032, 180 pp. https://www.datamintelligence.com/research-report/par-baked-bread-market

Ding, C., Cai, W. H., Sun, J. Y., Tao, H., & Wang, H. L. (2024). Unveiling the binding mechanism between starch granule-surface proteins and glutenins during dough mixing process. Food Hydrocolloids, 150, 109760. https://doi.org/10.1016/j.foodhyd.2024.109760

Fan, L., Wang, H., Li, M., Lei, M., Li, L., Ma, S., & Huang, J. (2024). Impact of wheat bran dietary fiber on gluten aggregation behavior in dough during noodle processing. International Journal of Biological Macromolecules, 257, Part 2, 128765. https://doi.org/10.1016/j.ijbiomac.2023.128765

Gaikwad, S., & Arya, S. S. (2018). Influence of frozen storage on quality of multigrain dough, par baked and ready to eat thalipeeth with additives. LWT, 96, 350-356. https://doi.org/10.1016/j.lwt.2018.05.057

Gerardo-Rodriguez, J. E., Ramírez-Wong B., Torres-Chávez, P. I. Ledesma-Osuna, A. I., Carvajal-Millán, E., López-Cervantes, J., & Silvas-García, M. I. (2021)- Effect of part-baking time, freezing rate and storage time on part-baked bread quality. Food Science and Technology, 41, 21. https://doi.org/10.1590/fst.06820

Halagarda, M. (2017). Effects of trehalose and dough additives incorporating enzymes on physical characteristics and sensory properties of frozen savory Danish dough. LWT, 86, 623-610. https://doi.org/10.1016/j.lwt.2017.08.048

Hong, T., Huo, X., Yang, T., Jin, Y., Xu, D., Wu, F., & Xu, X. (2023). Insights into zein on rheological, structural, thermal properties of wheat dough under glutathione and azodicarbonamide, salt, or acidic conditions. Food Bioscience, 56, 103329. https://doi.org/10.1016/j.fbio.2023.103329

Jiang, J., Yang, J., Fan, X., Zhang, Y., Li, M., Zhang, B., Guo, B., & Zhong, G. (2025). Regulation of ice crystal growth in frozen dough: From the effect of gluten and starch fractions interaction on water binding - A review. Food Chemistry, 476, 143509. https://doi.org/10.1016/j.foodchem.2025.143509

Li, B., Cao, Z., Zhang, W., Wei, S., Lv, Y., & Hu, Y. (2023). Protein oxidation-induced changes in the aggregation behavior and structure of gluten. LWT, 184, 115062. https://doi.org/10.1016/j.lwt.2023.115062

Li, H., Wang, J., Pan, L., & Lu, Q. (2019). Effect of amino and thiol groups of wheat gluten on the quality characteristics of Chinese noodles. Journal of Food Science and Technology, 56 (6), 2825-2835. https://doi.org/10.1007/s13197-019-03688-8

Lookhart, G. L., Bean, S. R., & Bietz, J. A. (2003). HPLC of gluten monomeric proteins. In P.R. Shewry and G.L. Lookhart (Eds.) Wheat Gluten Protein Analysis pp. 64–65, AACC. International, St. Paul, MN

Mahato, S., Zhu, Z., & Sun, D. W. (2019). Glass transitions as affected by food compositions and by conventional and novel freezing technologies: A review. Trends in Food Science & Technology, 94, 1-11. https://doi.org/10.1016/J.TIFS.2019.09.010

Marti, A., Marengo, M., Bonomi, F., Casiraghi, M. C., Franzetti, L., Pagani, M. A., & Iametti, S. (2017). Molecular features of fermented teff flour relate to its suitability for the production of enriched gluten-free bread. LWT - Food Science and Technology, 78, 296-302. https://doi.org/10.1016/j.lwt.2016.12.042

Osborne, T. B. (1907). Proteins of the wheat kernel. Washington: Carnegie Institution of Washington, 108–116. https://doi.org/10.5962/bhl.title.26152

Rani, M., Siddiqi, R. A., Sharma, R., Gill, B. S., & Sogi, D. S. (2023). Functional and structural properties of gliadin as influenced by pH, extraction protocols, and wheat cultivars. International Journal of Biological Macromolecules, 234, 123484. https://doi.org/10.1016/j.ijbiomac.2023.123484

Roos, Y. H. (2021). Glass transition and re-crystallization phenomena of frozen materials and their effect on frozen food quality. Foods, 10 (2), 447. https://doi.org/10.3390/foods10020447

Shu, Q., Wei, T., Liu, X., Liu, S., & Chen, Q. (2022). The dough-strengthening and spore-sterilizing effects of mannosylerythritol lipid-A in frozen dough and its application in bread making. Food Chemistry, 369, 131011. https://doi.org/10.1016/j.foodchem.2021.131011

Souza-Guimarães, D. J., Audino Zambelli, R., Amorim Afonso, M. R., & Ferreira Pontes, D. (2025). Cryoprotective potential of vegetable powders and polydextrose in frozen bread doughs. International Journal of Refrigeration, 172, 108-119. https://doi.org/10.1016/j.ijrefrig.2025.01.010

Su, T., Zhang, Y., Du, W., Zeng, J., & Gao, H. (2024). Effect of modified trehalose on regulation of gluten protein structure and steamed bread quality. International Journal of Biological Macromolecules, 283(4), 137969. https://doi.org/10.1016/j.ijbiomac.2024.137969

Taylor, J. R. N., Taylor, J., Campanella, O. H., & Hamaker, B. R. (2016). Functionality of the storage proteins in gluten-free cereals and pseudocereals in dough systems. Journal of Cereal Science, 67, 22-34. http://hdl.handle.net/2263/52232

Wei, Q., Zhang, G., & Xie, J. (2025). Modulatory effects of carboxymethyl chitosan on the textural and quality attributes of frozen rice dough and corresponding rice bread. Food Hydrocolloids, 162, 111021. https://doi.org/10.1016/j.foodhyd.2024.111021

Wieser, H., Koehler, P., & Scherf, K. A. (2023). Chemistry of wheat gluten proteins: Qualitative composition. Cereal Chemistry, 100(1), 23–35. https://doi.org/10.1002/cche.10572

Yang, T., Wang, P., Zhou, Q., Jiang, D., & Jiang, H. (2025). Effect of high-molecular-weight glutenin subunit deletion on gluten functionality and Chinese southern-type steamed bread quality. Food Chemistry, 464, 141664. https://doi.org/10.1016/j.foodchem.2024.141664

Yu, W., Xu, D., Zhang, H., Guo, L., Hong, T., Zhang, W., Jin, Y., & Xu, X. (2020). Effect of pigskin gelatin on baking, structural and thermal properties of frozen dough: Comprehensive studies on alteration of gluten network. Food Hydrocolloids, 102, 105591. https://doi.org/10.1016/j.foodhyd.2019.105591

Zhang, H., Fan, H., Xu, X., & Xu, D. (2024). Deterioration mechanisms and quality improvement methods in frozen dough: An updated review. Trends in Food Science & Technology, 143, 104251. https://doi.org/10.1016/j.tifs.2023.104251

Publicado

2025-11-18

Número

Sección

Alimentos

Cómo citar

Solubilidad de las proteínas del gluten en pan pre-horneado durante el almacenamiento en congelación. (2025). TECNOCIENCIA Chihuahua, 19, e2030. https://doi.org/10.54167/tch.v19iEspecial.2030